Anti-Human ATP5PO Polyclonal Antibody (HW696014)

Name: Anti-Human ATP5PO Polyclonal Antibody
Brand: abinScience
SKU: HW696014
Price: 96 USD
Availability: InStock

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SDS

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Número de catálogo

HW696014

Descripción

Anti-Human ATP5PO Polyclonal Antibody (HW696014) is a rabbit polyclonal antibody detecting ATP5PO in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ATP5PO (Gly36-Val213).

Objetivo

ATP synthase peripheral stalk subunit OSCP, ATP5O, Oligomycin sensitivity conferral protein, ATP5PO, OSCP, ATPO, ATP synthase subunit O, mitochondrial

Purificación

Purified by antigen affinity column.

Número de acceso

P48047

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

ATP synthase peripheral stalk subunit OSCP, mitochondrial (ATP5PO) is a ~23 kDa protein. Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-ATP5PO polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Hela cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse heart lysate
Lane 10: Mouse liver lysate
Lane11: Rat brain lysate
Lane12: Rat heart lysate
Lane13: Rat liver lysate

Referencias

Datasheet

Anti-Human ATP5PO Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human ATP5PO Polyclonal Antibody (HW696014)

Vista general

Imágenes

Referencias

Datasheet

SDS