Anti-Human ATP5PO Polyclonal Antibody (HW696014)

Name: Anti-Human ATP5PO Polyclonal Antibody
Brand: abinScience
SKU: HW696014
Price: 96 USD
Availability: InStock

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Каталожный номер

HW696014

Описание

Anti-Human ATP5PO Polyclonal Antibody (HW696014) is a rabbit polyclonal antibody detecting ATP5PO in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ATP5PO (Gly36-Val213).

Цель

ATP synthase peripheral stalk subunit OSCP, ATP5O, Oligomycin sensitivity conferral protein, ATP5PO, OSCP, ATPO, ATP synthase subunit O, mitochondrial

Очистка

Purified by antigen affinity column.

Номер доступа

P48047

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

ATP synthase peripheral stalk subunit OSCP, mitochondrial (ATP5PO) is a ~23 kDa protein. Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-ATP5PO polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Hela cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse heart lysate
Lane 10: Mouse liver lysate
Lane11: Rat brain lysate
Lane12: Rat heart lysate
Lane13: Rat liver lysate

Ссылки

Даташит

Anti-Human ATP5PO Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human ATP5PO Polyclonal Antibody (HW696014)

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Изображения

Ссылки

Даташит

SDS