Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Name: Anti-Human ATP6V1A Polyclonal Antibody
Brand: abinScience
SKU: HW292014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW292014

Descripción

Anti-Human ATP6V1A Polyclonal Antibody (HW292014) is a rabbit polyclonal antibody detecting ATP6V1A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ATP6V1A (Lys456-Asp617).

Objetivo

ATP6V1A1, ATP6A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69 kDa subunit, V-ATPase subunit A, ATP6V1A, Vacuolar proton pump subunit alpha, Vacuolar ATPase isoform VA68

Purificación

Purified by antigen affinity column.

Número de acceso

P38606

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

V-type proton ATPase catalytic subunit A (ATP6V1A) is a ~68 kDa protein. Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.

1. van Hille, B. et al. (1993) The Journal of biological chemistry 268, 7075-80. PMID: 8463241

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

4. Fassio, A. et al. (2018) Brain : a journal of neurology 141, 1703-1718. PMID: 29668857

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-ATP6V1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: U87 cell lysate
Lane 4: Daudi cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat brain lysate

Referencias

Datasheet

Anti-Human ATP6V1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Vista general

Imágenes

Referencias

Datasheet

SDS