Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Name: Anti-Human ATP6V1A Polyclonal Antibody
Brand: abinScience
SKU: HW292014
Price: 96 USD
Availability: InStock

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Каталожный номер

HW292014

Описание

Anti-Human ATP6V1A Polyclonal Antibody (HW292014) is a rabbit polyclonal antibody detecting ATP6V1A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Chicken, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ATP6V1A (Lys456-Asp617).

Цель

ATP6V1A1, ATP6A1, VPP2, V-type proton ATPase catalytic subunit A, V-ATPase 69 kDa subunit, V-ATPase subunit A, ATP6V1A, Vacuolar proton pump subunit alpha, Vacuolar ATPase isoform VA68

Очистка

Purified by antigen affinity column.

Номер доступа

P38606

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

V-type proton ATPase catalytic subunit A (ATP6V1A) is a ~68 kDa protein. Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.

1. van Hille, B. et al. (1993) The Journal of biological chemistry 268, 7075-80. PMID: 8463241

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

4. Fassio, A. et al. (2018) Brain : a journal of neurology 141, 1703-1718. PMID: 29668857

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-ATP6V1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: U87 cell lysate
Lane 4: Daudi cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse kidney lysate
Lane 8: Rat brain lysate

Ссылки

Даташит

Anti-Human ATP6V1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human ATP6V1A Polyclonal Antibody (HW292014)

Обзор

Изображения

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