Anti-Human ATP6V1B2 Polyclonal Antibody (HB120014)

Name: Anti-Human ATP6V1B2 Polyclonal Antibody
Brand: abinScience
SKU: HB120014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB120014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ATP6V1B2 (Ser51-His511).

Cible

Endomembrane proton pump 58 kDa subunit, ATP6B2, V-type proton ATPase subunit B, brain isoform, HO57, VPP3, Vacuolar proton pump subunit B 2, ATP6V1B2, V-ATPase subunit B 2

Purification

Purified by antigen affinity column.

Numéro d'accès

P21281

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

V-type proton ATPase subunit B, brain isoform (ATP6V1B2) is a ~56 kDa protein. Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.

1. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ATP6V1B2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse brain lysate
Lane 4: Rat kidney lysate
Lane 5: Rat brain lysate

Reférences

Fiche technique

Anti-Human ATP6V1B2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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