Anti-Human ATP6V1G1 Polyclonal Antibody (HF937014)

Name: Anti-Human ATP6V1G1 Polyclonal Antibody
Brand: abinScience
SKU: HF937014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
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Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HF937014

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ATP6V1G1 (Met1-Gly118).

Objetivo

ATP6V1G1, Vacuolar proton pump subunit M16, ATP6G, V-type proton ATPase subunit G 1, Vacuolar proton pump subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, ATP6G1, ATP6J

Purificación

Purified by antigen affinity column.

Número de acceso

O75348

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

V-type proton ATPase subunit G 1 (ATP6V1G1) is a ~13 kDa protein. Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.

1. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

2. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human ATP6V1G1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo