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Обзор
Каталожный номерHV444014
Описание
Anti-Human AUP1 Polyclonal Antibody (HV444014) is a rabbit polyclonal antibody detecting AUP1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human AUP1 (Val201-Asp410).
Цель AUP1
Очистка Purified by antigen affinity column.
Номер доступа Q9Y679
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияLipid droplet-regulating VLDL assembly factor AUP1, Ancient ubiquitous protein 1, AUP1
Фон

Lipid droplet-regulating VLDL assembly factor AUP1 is a ~45 kDa protein. Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2.

1. Mueller, B. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12325-30. PMID: 18711132
2. Klemm, EJ. et al. (2011) The Journal of biological chemistry 286, 37602-14. PMID: 21857022
3. Lohmann, D. et al. (2013) PloS one 8, e72453. PMID: 24039768
4. Spandl, J. et al. (2011) The Journal of biological chemistry 286, 5599-606. PMID: 21127063
5. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569
6. Zhang, J. et al. (2017) Arteriosclerosis, thrombosis, and vascular biology 37, 633-642. PMID: 28183703
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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