Anti-Human Axl Recombinant Antibody (SAA0128) (HB709207)

Name: Anti-Human Axl Recombinant Antibody (SAA0128)
Brand: abinScience
SKU: HB709207
Price: 191 USD
Availability: InStock

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Numéro de catalogue	HB709207
Reactivité des espèces	Human
Applications	FCM, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, kappa
ID de clone	SAA0128
Applications testées	FCM
Cible	UFO, AXL oncogene, Tyrosine-protein kinase receptor UFO, AXL
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P30530
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Tyrosine-protein kinase receptor UFO (AXL) is a ~98 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase.
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human AXL antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AXL antibody monoclonal antibody (Catalog # HB709207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human AXL antibody.U251 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AXL antibody monoclonal antibody (Catalog # HB709207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.