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Anti-Human B3GAT3 Polyclonal Antibody (HF746014)

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Aperçu
Numéro de catalogueHF746014
Description
Anti-Human B3GAT3 Polyclonal Antibody (HF746014) is a rabbit polyclonal antibody detecting B3GAT3 in ELISA, IHC, WB. Suitable for Cricetulus griseus, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human B3GAT3 (Leu74-Val335).
Cible B3GAT3
Purification Purified by antigen affinity column.
Numéro d'accès O94766
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, 2.4.1.135, Beta-1, 3-glucuronyltransferase 3, Glucuronosyltransferase I, GlcAT-I, UDP-GlcUA:Gal beta-1, 3-Gal-R glucuronyltransferase, GlcUAT-I, B3GAT3
Contexte

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is a ~37 kDa protein. Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.

1. Budde, BS. et al. (2015) Human genetics 134, 691-704. PMID: 25893793
2. Koike, T. et al. (2014) The Journal of biological chemistry 289, 6695-6708. PMID: 24425863
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human B3GAT3 Polyclonal Antibody

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