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Anti-Human BAAT Polyclonal Antibody (HA552014)

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Vista general
Número de catálogoHA552014
Descripción
Anti-Human BAAT Polyclonal Antibody (HA552014) is a rabbit polyclonal antibody detecting BAAT in WB, IHC, ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesWB, IHC, ELISA
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human BAAT (Met1-Leu418).
Objetivo BAAT
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso Q14032
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosBile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, EC:2.3.1.65, Bile acid-CoA thioesterase, Choloyl-CoA hydrolase, EC:3.1.2.27, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, EC:3.1.2.2, BAAT
Fondo

Bile acid-CoA:amino acid N-acyltransferase (BAAT) is a ~46 kDa protein. Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine. More than 95% of the BAs are N-acyl amidates with glycine and taurine. Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism. This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption. May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

1. Sfakianos, MK. et al. (2002) The Journal of biological chemistry 277, 47270-5. PMID: 12239217
2. O'Byrne, J. et al. (2003) The Journal of biological chemistry 278, 34237-44. PMID: 12810727
3. Johnson, MR. et al. (1991) The Journal of biological chemistry 266, 10227-33. PMID: 2037576
4. Falany, CN. et al. (1994) The Journal of biological chemistry 269, 19375-9. PMID: 8034703
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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