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Anti-Human BAAT Polyclonal Antibody (HA552014)

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Aperçu
Numéro de catalogueHA552014
Description
Anti-Human BAAT Polyclonal Antibody (HA552014) is a rabbit polyclonal antibody detecting BAAT in WB, IHC, ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsWB, IHC, ELISA
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human BAAT (Met1-Leu418).
Cible BAAT
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès Q14032
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsBile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, EC:2.3.1.65, Bile acid-CoA thioesterase, Choloyl-CoA hydrolase, EC:3.1.2.27, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, EC:3.1.2.2, BAAT
Contexte

Bile acid-CoA:amino acid N-acyltransferase (BAAT) is a ~46 kDa protein. Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine. More than 95% of the BAs are N-acyl amidates with glycine and taurine. Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism. This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption. May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

1. Sfakianos, MK. et al. (2002) The Journal of biological chemistry 277, 47270-5. PMID: 12239217
2. O'Byrne, J. et al. (2003) The Journal of biological chemistry 278, 34237-44. PMID: 12810727
3. Johnson, MR. et al. (1991) The Journal of biological chemistry 266, 10227-33. PMID: 2037576
4. Falany, CN. et al. (1994) The Journal of biological chemistry 269, 19375-9. PMID: 8034703
Note For research use only.
Images
Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human BAAT Polyclonal Antibody

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