Anti-Human BHLHE40 Polyclonal Antibody (HT136014)

Name: Anti-Human BHLHE40 Polyclonal Antibody
Brand: abinScience
SKU: HT136014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HT136014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human BHLHE40 (Ser46-Gly186).

Cible

DEC1, bHLHe40, Differentially expressed in chondrocytes protein 1, Class E basic helix-loop-helix protein 40, Class B basic helix-loop-helix protein 2, STRA13, SHARP2, SHARP-2, bHLHb2, Enhancer-of-split and hairy-related protein 2, BHLHB2, BHLHE40, Stimulated by retinoic acid gene 13 protein

Purification

Purified by antigen affinity column.

Numéro d'accès

O14503

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Class E basic helix-loop-helix protein 40 (BHLHE40) is a ~45 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.

1. Honma, S. et al. (2002) Nature 419, 841-4. PMID: 12397359

2. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297

3. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706

4. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144

5. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782

6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558

7. Marczak, MM. et al. (2017) Archives of biochemistry and biophysics 631, 11-18. PMID: 28797635

8. Nakashima, A. et al. (2018) Hypertension (Dallas, Tex. : 1979) 72, 746-754. PMID: 30012868

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human BHLHE40 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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