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Anti-Human BNIP1 Polyclonal Antibody (HD183014)

Anti-Human BNIP1 Polyclonal Antibody
Anti-Human BNIP1 Polyclonal Antibody
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  • 100ug
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Aperçu
Numéro de catalogueHD183014
Description
Anti-Human BNIP1 Polyclonal Antibody (HD183014) is a rabbit polyclonal antibody detecting BNIP1. Suitable for Mouse, Rat, and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human BNIP1 (Met1-Leu199).
Cible BCL2/adenovirus E1B 19 kDa protein-interacting protein 1, BNIP1, NIP1, SEC20L, TRG-8, Transformation-related gene 8 protein, Vesicle transport protein SEC20
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q12981
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Vesicle transport protein SEC20 (BNIP1) is a ~26 kDa protein. As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).

1. Nakajima, K. et al. (2004) The EMBO journal 23, 3216-26. PMID: 15272311
2. Wang, P. et al. (2013) Cellular signalling 25, 2320-33. PMID: 23896122
3. Boyd, JM. et al. (1994) Cell 79, 341-51. PMID: 7954800
Note For research use only
Images
  • Anti-Human BNIP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-BNIP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: PC-12 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: Mouse heart lysate
    Lane 6: Mouse skeletal muscle lysate
    Lane 7: Mouse testis lysate
    Lane 8: Rat brain lysate
    Lane 9: Rat heart lysate
    Lane 10: Rat testis lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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