中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HF757013 | ||||
|---|---|---|---|---|---|
| 種反応性 | Human | ||||
| アプリケーション | ELISA, IF, IP, IP-MS | ||||
| 宿主種 | Mouse | ||||
| クローン性 | Monoclonal | ||||
| アイソタイプ | IgG2a, kappa | ||||
| クローンID | SAA0192 | ||||
| ターゲット | Protein HUNK1, Bromodomain-containing protein 4, BRD4, HUNK1 | ||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||
| 純度 | >95% as determined by SDS-PAGE. | ||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||
| アクセッション番号 | O60885 | ||||
| RRID | Anti-Human BRD4 IP Antibody (SAA0192) (abinScience Cat# HF757013, RRID:AB_3729726) |
||||
| 形態 | Liquid | ||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||
| 製品使用情報 |
| ||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Bromodomain-containing protein 4 (BRD4) is a ~152 kDa protein. Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. 1. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596 2. Zhang, W. et al. (2012) The Journal of biological chemistry 287, 43137-55. PMID: 23086925 3. Wu, SY. et al. (2013) Molecular cell 49, 843-57. PMID: 23317504 4. Konuma, T. et al. (2017) Scientific reports 7, 16272. PMID: 29176719 5. Olley, G. et al. (2018) Nature genetics 50, 329-332. PMID: 29379197 6. Wang, R. et al. (2012) The Journal of biological chemistry 287, 10738-52. PMID: 22334664 7. Patel, MC. et al. (2013) Molecular and cellular biology 33, 2497-507. PMID: 23589332 8. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376 9. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377 | ||||
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Human BRD4 IP Antibody (SAA0192).
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
