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Anti-Human Complement C5 Polyclonal Antibody (HF687024)

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Vista general
Número de catálogoHF687024
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human C5 (Asp822-Lys925).
Objetivo CPAMD4, C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5
Purificación Purified by antigen affinity column.
Número de acceso P01031
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Complement C5 is a ~188 kDa protein. Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways.

1. Rawal, N. et al. (2003) The Journal of biological chemistry 278, 38476-83. PMID: 12878586
2. Rawal, N. et al. (2008) The Journal of biological chemistry 283, 7853-63. PMID: 18204047
3. Heesterbeek, DA. et al. (2019) The EMBO journal 38. PMID: 30643019
4. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279
5. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456
6. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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