Anti-Human C8A Polyclonal Antibody (HY173014)

Name: Anti-Human C8A Polyclonal Antibody
Brand: abinScience
SKU: HY173014
Price: 168 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HY173014

Description

Anti-Human C8A Polyclonal Antibody (HY173014) is a rabbit polyclonal antibody detecting C8A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human C8A (Ala135-Arg498).

Cible

Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A

Purification

Purified by antigen affinity column.

Numéro d'accès

P07357

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Complement component C8 alpha chain (C8A) is a ~65 kDa protein. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C8A, together with C8B and C8G, inserts into the target membrane, but does not form pores by itself. During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity.

1. Hadders, MA. et al. (2007) Science (New York, N.Y.) 317, 1552-4. PMID: 17872444

2. Hadders, MA. et al. (2012) Cell reports 1, 200-7. PMID: 22832194

3. Serna, M. et al. (2016) Nature communications 7, 10587. PMID: 26841837

4. Sharp, TH. et al. (2016) Cell reports 15, 1-8. PMID: 27052168

5. Menny, A. et al. (2018) Nature communications 9, 5316. PMID: 30552328

6. Steckel, EW. et al. (1980) The Journal of biological chemistry 255, 11997-2005. PMID: 7440581

7. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456

8. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882

9. Steckel, EW. et al. (1983) The Journal of biological chemistry 258, 4318-24. PMID: 6833260

Note

For research use only.

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Anti-Human C8A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human C8A Polyclonal Antibody (HY173014)

Aperçu

Images

Reférences

Fiche technique

SDS