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Anti-Human CA4 Polyclonal Antibody (HB731014)

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概要
カタログ番号HB731014
説明
Anti-Human CA4 Polyclonal Antibody (HB731014) is a rabbit polyclonal antibody detecting CA4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Human
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human CA4 (Ala19-Lys283).
ターゲット Carbonic anhydrase 4, Carbonate dehydratase IV, CA-IV, CA4, Carbonic anhydrase IV
精製 Purified by antigen affinity column.
アクセッション番号 P22748
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Carbonic anhydrase 4 (CA4) is a ~35 kDa protein. Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

1. Yang, Z. et al. (2005) Human molecular genetics 14, 255-65. PMID: 15563508
2. Temperini, C. et al. (2006) Journal of medicinal chemistry 49, 3019-27. PMID: 16686544
3. Temperini, C. et al. (2006) Chemistry (Weinheim an der Bergstrasse, Germany) 12, 7057-66. PMID: 16807956
4. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 628-35. PMID: 17127057
5. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 2210-5. PMID: 17314045
6. Alvarez, BV. et al. (2007) Investigative ophthalmology & visual science 48, 3459-68. PMID: 17652713
7. Köhler, K. et al. (2007) Angewandte Chemie (International ed. in English) 46, 7697-9. PMID: 17705204
8. Di Fiore, A. et al. (2009) Proteins 74, 164-75. PMID: 18618712
9. Crocetti, L. et al. (2009) Bioorganic & medicinal chemistry letters 19, 1371-5. PMID: 19186056
10. Maresca, A. et al. (2009) Journal of the American Chemical Society 131, 3057-62. PMID: 19206230
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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