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| カタログ番号 | HP765014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human CARM1 Polyclonal Antibody (HP765014) is a rabbit polyclonal antibody detecting CARM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CARM1 (Thr28-Asn471). | ||||||||
| ターゲット | Histone-arginine methyltransferase CARM1, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4, Protein arginine N-methyltransferase 4 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q86X55 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Histone-arginine methyltransferase CARM1 is a ~65 kDa protein. Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. 1. Li, H. et al. (2002) The Journal of biological chemistry 277, 44623-30. PMID: 12237300 2. Miao, F. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 1562-73. PMID: 16497732 3. Lakowski, TM. et al. (2009) The Biochemical journal 421, 253-61. PMID: 19405910 4. Lee, YH. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 3611-6. PMID: 15731352 5. Genois, MM. et al. (2021) Molecular cell 81, 784-800.e8. PMID: 33412112 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-CARM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate
Lane 2: Rat liver lysate
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