Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014)

Name: Anti-Human CASP3/Caspase-3 Polyclonal Antibody
Brand: abinScience
SKU: HW555014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HW555014

Description

Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014) is a rabbit polyclonal antibody detecting Caspase-3 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, Dog, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human CASP3/Caspase-3 (Ser29-His277).

Cible

Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P42574, P55213, Q95ND5, Q8MKI5, P70677, Q8MJU1, Q8MJC3

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).

1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680

2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630

3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375

4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 7596430

10. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CASP3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Reférences

Fiche technique

Anti-Human CASP3/Caspase-3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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