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| カタログ番号 | HW555014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014) is a rabbit polyclonal antibody detecting Caspase-3 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, Dog, and Mouse.
Highlights
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| 種反応性 | Human, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CASP3/Caspase-3 (Ser29-His277). | ||||||||
| ターゲット | Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P42574, P55213, Q95ND5, Q8MKI5, P70677, Q8MJU1, Q8MJC3 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). 1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680 2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630 3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375 4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844 5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120 6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 7596430 10. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-CASP3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
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