Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014)

Name: Anti-Human CASP3/Caspase-3 Polyclonal Antibody
Brand: abinScience
SKU: HW555014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HW555014

Описание

Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014) is a rabbit polyclonal antibody detecting Caspase-3 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, Dog, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human CASP3/Caspase-3 (Ser29-His277).

Цель

Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P42574, P55213, Q95ND5, Q8MKI5, P70677, Q8MJU1, Q8MJC3

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).

1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680

2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630

3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375

4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 7596430

10. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-CASP3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

Ссылки

Даташит

Anti-Human CASP3/Caspase-3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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