Anti-Human CBX4 Recombinant Antibody (P004) (HT334013)

Name: Anti-Human CBX4 Recombinant Antibody (P004)
Brand: abinScience
SKU: HT334013
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HT334013

Reactividad de especies

Human

Aplicaciones

ELISA, IF, IP

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a

ID de clon

P004

Objetivo

CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

O00257

RRID

Anti-Human CBX4 Antibody (P004) (abinScience Cat# HT334013, RRID:AB_3729487)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

E3 SUMO-protein ligase CBX4 is a ~61 kDa protein. E3 SUMO-protein ligase that catalyzes sumoylation of target proteins by promoting the transfer of SUMO from the E2 enzyme to the substrate. Also acts as a histone reader, which specifically recognizes and binds histone H3 trimethylated at 'Lys-9' and 'Lys-27' (H3K9me3 and H3K27me3, respectively) via its chromo domain. Catalyzes sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Acts as a regulator of brown adipocyte differentiation by mediating sumoylation of PRDM16, thereby preventing PRDM16 ubiquitination and degradation. Monosumoylates ZNF131.

1. Kagey, MH. et al. (2003) Cell 113, 127-37. PMID: 12679040

2. Oh, Y. et al. (2012) The Journal of biological chemistry 287, 17517-17529. PMID: 22467880

3. Pelisch, F. et al. (2012) The Journal of biological chemistry 287, 30789-99. PMID: 22825850

4. Levine, SS. et al. (2002) Molecular and cellular biology 22, 6070-8. PMID: 12167701

5. Maertens, GN. et al. (2009) PloS one 4, e6380. PMID: 19636380

6. Vandamme, J. et al. (2011) Molecular & cellular proteomics : MCP 10, M110.002642. PMID: 21282530

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human CBX4 Antibody (P004)

Referencias

Datasheet

Anti-Human CBX4 Recombinant Antibody (P004).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo