Anti-Human CCL20/MIP-3-alpha Recombinant Antibody (SAA0277) (HX131013)

Name: Anti-Human CCL20/MIP-3-alpha Recombinant Antibody (SAA0277)
Brand: abinScience
SKU: HX131013
Price: 110 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HX131013

Reactividad de especies

Human

Aplicaciones

Blocking, ELISA, FCM

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

SAA0277

Objetivo

Beta-chemokine exodus-1, SCYA20, LARC, C-C motif chemokine 20, Liver and activation-regulated chemokine, Small-inducible cytokine A20, MIP-3-alpha, CCL20, MIP3A, Macrophage inflammatory protein 3 alpha, CC chemokine LARC

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P78556

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

C-C motif chemokine 20 (CCL20/MIP-3-alpha) is a ~10 kDa protein. Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation.

1. Schutyser, E. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 165, 4470-7. PMID: 11035086

2. Nelson, RT. et al. (2001) Genomics 73, 28-37. PMID: 11352563

3. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036

4. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174

5. Hieshima, K. et al. (1997) The Journal of biological chemistry 272, 5846-53. PMID: 9038201

6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988

7. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636

8. Hromas, R. et al. (1997) Blood 89, 3315-22. PMID: 9129037

Nota

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

Imágenes

Flow Cytometry

Flow-cytometry using anti-human CCL20 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL20 antibody monoclonal antibody (Catalog # HX131013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CCL20/MIP-3-alpha Antibody (SAA0277).