Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539) (HX155107)

Name: Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539)
Brand: abinScience
SKU: HX155107
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HX155107
Reactivité des espèces	Human
Applications	ELISA, FCM, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, kappa
ID de clone	SAA0539
Cible	C-C motif chemokine 7, MCP-3, CCL7, Monocyte chemotactic protein 3, Monocyte chemoattractant protein 3, NC28, Small-inducible cytokine A7, SCYA6, SCYA7, MCP3
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P80098
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.09% Sodium azide. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	C-C motif chemokine 7 (CCL7/MCP-3) is a ~11 kDa protein. Chemotactic factor that plays an important role in immune regulation. Attracts monocytes, eosinophils, basophils, and T-cells to sites of inflammation or infection. Upon binding to various chemokine receptors including CCR1, CCR2, CCR3, and CCR5, facilitates immune cell migration by guiding them to infected tissues. Interacts with CCR2 to facilitate the release of monocytes from the bone marrow into the bloodstream, maintaining monocyte homeostasis. In turn, monocytes recruited to inflamed or injured tissues can differentiate into macrophages or dendritic cells, which are essential for immune defense and tissue repair. 1. Bonini, JA. et al. (1997) DNA and cell biology 16, 1249-56. PMID: 9364936 2. Blanpain, C. et al. (1999) Blood 94, 1899-905. PMID: 10477718 3. Wei, H. et al. (2024) Cellular signalling 117, 111122. PMID: 38417634
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CCL7 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL7 antibody monoclonal antibody (Catalog # HX155107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CCL24/Eotaxin-2/MPIF-2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CCL24/Eotaxin-2/MPIF-2 (Catalog No: HT048012)
Lane 2: negative control

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Anti-Human CCL7/MCP-3 Recombinant Antibody (SAA0539).pdf

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