Anti-Human CD16/FcgR3 Nanobody (SAA2149) [SAA2149] (HY386023) is a alpaca monoclonal antibody detecting CD16-I in Blocking, ELISA. Suitable for Human.
Реактивность видов
Human
Применения
Blocking, ELISA, WB
Хозяинский вид
Alpaca
Клоональность
Monoclonal
Изотип
VHH-8His-Cys-tag
ID клона
SAA2149
Проверенные приложения
WB
Цель
Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, CD16-I, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3, Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>95% as determined by SDS-PAGE.
Очистка
Purified by Nickel column.
Номер доступа
P08637, O75015
Форма
Liquid
Буфер хранения
0.01M PBS pH 7.4
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Информация об использовании продукта
Применение
Разбавление
ELISA
1:2000-1:20000
WB
1:500-1:2000
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон
Low affinity immunoglobulin gamma Fc region receptor III-A (CD16/FcgR3) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.
1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607
2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335
3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369
4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922
5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175
6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339
7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325
8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432
SDS-PAGE for Anti-Human CD16/FcgR3 Nanobody (SAA2149).
Bioactivity
Detects recombinant CD16b (Catalog No: HF886011) in indirect ELISA.
Western blot
Western blot analysis was performed using anti-CD16b monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD16b (Catalog No: HF886011)
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