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Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC (HB243117)

Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC
Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC
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Vista general
Número de catálogoHB243117
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clon3H9
ConjugaciónFITC
Objetivo IL8RB, GRO/MGSA receptor, C-X-C chemokine receptor type 2, CD182, CXCR-2, IL-8R B, CXC-R2, IL-8 receptor type 2, High affinity interleukin-8 receptor B, CDw128b, CXCR2
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Número de acceso P25025
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

C-X-C chemokine receptor type 2 (CD182/CXCR2) is a ~40 kDa protein. Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.

1. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716
2. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD182 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD182 monoclonal antibody (Catalog: HB243117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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