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Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC (HB243117)

Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC
Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC
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  • 100T
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Aperçu
Numéro de catalogueHB243117
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de clone3H9
ConjugaisonFITC
Cible IL8RB, GRO/MGSA receptor, C-X-C chemokine receptor type 2, CD182, CXCR-2, IL-8R B, CXC-R2, IL-8 receptor type 2, High affinity interleukin-8 receptor B, CDw128b, CXCR2
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Numéro d'accès P25025
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

C-X-C chemokine receptor type 2 (CD182/CXCR2) is a ~40 kDa protein. Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.

1. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716
2. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CXCR2/IL-8RB Recombinant Antibody (3H9), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD182 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD182 monoclonal antibody (Catalog: HB243117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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