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Anti-Human CD20 Recombinant Antibody (SAA0007), PE
Anti-Human CD20 Recombinant Antibody (SAA0007), PE
Цена(USD):
Спецификация:
  • 50T
  • 100T
Количество:
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  • Обзор

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  • Даташит

  • SDS

Обзор
Каталожный номерHY257227
Реактивность видовHuman
ПримененияFCM
Хозяинский видMouse
КлоональностьMonoclonal
ИзотипIgG2b, kappa
ID клонаSAA0007
КонъюгацияPE
Проверенные приложения FCM
Цель B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Связанные продукты Mouse IgG2b, kappa Recombinant Isotype Control Antibody (MPC-11), PE
Номер доступа P11836
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Устойчивость и хранение Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон

B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva).

1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111
2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015
3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739
4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602
Примечание For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Изображения
  • Anti-Human CD20 Recombinant Antibody (SAA0007), PE

    Flow Cytometry

    Flow-cytometry using PE anti-human CD20 antibody. Daudi cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD20 antibody monoclonal antibody (Catalog # HY257227, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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