Anti-Human IGF-I R/IGF1R Recombinant Antibody (2.13.2#) (HY353207)

Name: Anti-Human IGF-I R/IGF1R Recombinant Antibody (2.13.2#)
Brand: abinScience
SKU: HY353207
Price: 191 USD
Availability: InStock

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Numéro de catalogue	HY353207
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG2, kappa
ID de clone	2.13.2#
Applications testées	FCM
Cible	CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P08069
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins.
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD221/IGF1R Antibody (2.13.2#).
Flow Cytometry

Flow-cytometry using anti-human CD221 antibody. Human HeLa cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD221 antibody monoclonal antibody (Catalog # HY353207 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD221 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD221 antibody monoclonal antibody (Catalog # HY353207 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.