



| Número de catálogo | HB102407 |
|---|---|
| Reactividad de especies | Human |
| Aplicaciones | ELISA, FCM |
| Especie huésped | Human |
| Clonalidad | Monoclonal |
| Isotipo | IgG1, kappa |
| ID de clon | SAA2216 |
| Aplicaciones probadas | FCM |
| Objetivo | Breast carcinoma-associated antigen DF3, CA 15-3, CD227, Cancer antigen 15-3, Carcinoma-associated mucin, EMA, Episialin, H23AG, KL-6, Krebs von den Lungen-6, MUC-1, MUC1, MUC1-CT, MUC1-NT, MUC1-alpha, MUC1-beta, Mucin-1, Mucin-1 subunit alpha, Mucin-1 subunit beta, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin |
| Nivel de endotoxinas | < 10 EU/mg |
| Pureza | >95% as determined by SDS-PAGE. |
| Purificación | Protein A/G purified from cell culture supernatant. |
| Número de acceso | P15941 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Fondo | Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
| Nota | For research use only. |

Flow-cytometry using anti-human CD227 antibody. MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD227 antibody. CD227 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.




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