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Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43), FITC (HY094117)

Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43), FITC
Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43), FITC
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Vista general
Número de catálogoHY094117
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonsc57.43
ConjugaciónFITC
Aplicaciones probadas FCM
Objetivo CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Número de acceso P08582
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

Melanotransferrin (CD228/MELTF) is a ~80 kDa protein. Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD228 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD228 monoclonal antibody (Catalog: HY094117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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