Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43) (HY094107)

Name: Anti-Human Melanotransferrin/CD228/MFI2 Recombinant Antibody (sc57.43)
Brand: abinScience
SKU: HY094107
Price: 191 USD
Availability: InStock

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概要
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参考文献
データシート
SDS

概要

カタログ番号	HY094107
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	sc57.43
テスト済みアプリケーション	FCM
ターゲット	CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P08582
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Melanotransferrin (CD228/MELTF) is a ~80 kDa protein. Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
注意事項	For research use only.

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Bioactivity

Detects CD228/MELTF in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD228 antibody.A375 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD228 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.