





| Каталожный номер | HY094107 |
|---|---|
| Реактивность видов | Human |
| Применения | FCM |
| Хозяинский вид | Mouse |
| Клоональность | Monoclonal |
| Изотип | IgG1, kappa |
| ID клона | sc57.43 |
| Проверенные приложения | FCM |
| Цель | CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >95% as determined by SDS-PAGE. |
| Очистка | Protein A/G purified from cell culture supernatant. |
| Номер доступа | P08582 |
| Форма | Liquid |
| Буфер хранения | 0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Фон | Melanotransferrin (CD228/MELTF) is a ~80 kDa protein. Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. |
| Примечание | For research use only. |

Detects CD228/MELTF in indirect ELISAs.

Flow-cytometry using anti-human CD228 antibody.A375 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD228 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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