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Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC (HF996117)

Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC
Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC
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Vista general
Número de catálogoHF996117
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clon1H4
ConjugaciónFITC
Aplicaciones probadas FCM
Objetivo CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Productos asociados Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Número de acceso P01589
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC

    Flow cytometry

    Flow-cytometry FITC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD25 monoclonal antibody (Catalog HF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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