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Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC (HF996117)

Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC
Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC
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  • 100T
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Aperçu
Numéro de catalogueHF996117
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de clone1H4
ConjugaisonFITC
Applications testées FCM
Cible CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Produits associés Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Numéro d'accès P01589
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), FITC

    Flow cytometry

    Flow-cytometry FITC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD25 monoclonal antibody (Catalog HF996117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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