Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F5), APC (HB796337)

Name: Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F5), APC
Brand: abinScience
SKU: HB796337
Price: 290 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HB796337
種反応性	Human
アプリケーション	FCM
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	1F5
抱合	APC
テスト済みアプリケーション	FCM
ターゲット	CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
エンドトキシンレベル	Please contact with the lab for this information.
関連製品	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
精製	Affinity chromatography.
アクセッション番号	P26842
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Human CD27 antibody. Raji cells were stained with an irrelevant antibody (Red Histogram) or an APC anti-Human CD27 antibody monoclonal antibody (Catalog # HB796337 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献

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