Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F5), APC (HB796337)

Name: Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F5), APC
Brand: abinScience
SKU: HB796337
Price: 290 USD
Availability: InStock

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Каталожный номер	HB796337
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	1F5
Конъюгация	APC
Проверенные приложения	FCM
Цель	CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
Уровень эндотоксинов	Please contact with the lab for this information.
Связанные продукты	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Очистка	Affinity chromatography.
Номер доступа	P26842
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Store at 4°C for 12 months. Protect from light. Do not freeze.
Фон	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Примечание	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-Human CD27 antibody. Raji cells were stained with an irrelevant antibody (Red Histogram) or an APC anti-Human CD27 antibody monoclonal antibody (Catalog # HB796337 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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