Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24) (HB796207)

Name: Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (5F24)
Brand: abinScience
SKU: HB796207
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB796207
Reactivité des espèces	Human
Applications	FCM, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, kappa
ID de clone	5F24
Applications testées	WB, FCM
Cible	CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P26842
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.09% Sodium azide. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Note	For research use only.

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Bioactivity

Detects Human CD27 in indirect ELISAs.
Flow CytoMetry

Flow-cytometry using anti-human CD27 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD27 monoclonal antibody (Catalog: HB796207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD27 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant human CD27 (Catalog No: HB796011)
Lane 2: negative control