Anti-Human ICOS Recombinant Antibody (SAA0092) (HV702207)

Name: Anti-Human ICOS Recombinant Antibody (SAA0092)
Brand: abinScience
SKU: HV702207
Price: 191 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HV702207
種反応性	Human
アプリケーション	FCM
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG4, kappa
クローンID	SAA0092
テスト済みアプリケーション	FCM
ターゲット	CD278, ICOS, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	Q9Y6W8
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Inducible T-cell costimulator (CD278/ICOS) is a ~22 kDa protein. Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. 1. Hutloff, A. et al. (1999) Nature 397, 263-6. PMID: 9930702 2. Rujas, E. et al. (2020) Nature communications 11, 5066. PMID: 33033255 3. Pedros, C. et al. (2016) Nature immunology 17, 825-33. PMID: 27135603 4. Wan, Z. et al. (2020) Cellular & molecular immunology 17, 143-152. PMID: 30523347
注意事項	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD278 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD278 antibody monoclonal antibody (Catalog # HV702207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD278 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD278 monoclonal antibody (Catalog: HV702207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.