Anti-Human CD28 Recombinant Antibody (SAA0010), PE (HY422127)

Name: Anti-Human CD28 Recombinant Antibody (SAA0010), PE
Brand: abinScience
SKU: HY422127
Price: 256 USD
Availability: InStock

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Número de catálogo	HY422127
Reactividad de especies	Human
Aplicaciones	ELISA, FCM, WB
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG4
ID de clon	SAA0010
Conjugación	PE
Aplicaciones probadas	FCM
Objetivo	CD28, TP44, T-cell-specific surface glycoprotein CD28, RHC83401
Nivel de endotoxinas	Please contact with the lab for this information.
Productos asociados	Human IgG4 (S228P) Recombinant Isotype Control Antibody (13R4), PE
Número de acceso	P10747
Forma	Liquid
Buffer de almacenamiento	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. 1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475 2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038 3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965 4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202 6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608 7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using PE anti-human CD28 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-human CD28 antibody monoclonal antibody (Catalog # HY422127, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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Datasheet