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Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3) (HY139107)

Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHY139107
Reactividad de especiesHuman
AplicacionesELISA, FCM, IF, IP
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonIV.3
Aplicaciones probadas FCM
Objetivo Fc-gamma RII-a, IgG Fc receptor II-a, IGFR2, FCG2, CDw32, FCGR2A1, FCGR2A, FcRII-a, CD32, Low affinity immunoglobulin gamma Fc region receptor II-a, Fc-gamma-RIIa
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P12318
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Low affinity immunoglobulin gamma Fc region receptor II-a (CD32/FCGR2A) is a ~35 kDa protein. Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

Nota For research use only.
Imágenes
  • Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)

    Flow Cytometry

    Flow-cytometry using anti-human CD32 antibody.CD32 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32 antibody monoclonal antibody (Catalog # HY139107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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