Anti-Human E-Cadherin Recombinant Antibody (SAA0269) (HY460207)

Name: Anti-Human E-Cadherin Recombinant Antibody (SAA0269)
Brand: abinScience
SKU: HY460207
Price: 191 USD
Availability: InStock

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Número de catálogo	HY460207
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	SAA0269
Aplicaciones probadas	FCM
Objetivo	CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P12830
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. 1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333 2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492
Nota	For research use only.

Imágenes

Flow Cytometry

Flow-cytometry using anti-human CD324 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD324 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD324 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD324 monoclonal antibody (Catalog: HY460207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.