Anti-Human CD34 Recombinant Antibody (My10) (HB766107)

Name: Anti-Human CD34 Recombinant Antibody (My10)
Brand: abinScience
SKU: HB766107
Price: 196 USD
Availability: InStock

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Vista general

Número de catálogo	HB766107
Reactividad de especies	Human
Aplicaciones	FCM, WB
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	My10
Aplicaciones probadas	FCM
Objetivo	CD34, Hematopoietic progenitor cell antigen CD34
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P28906
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Nota	For research use only.

Imágenes

SEC-HPLC

SEC-HPLC detection for Anti-Human CD34 Antibody (My10).
Bioactivity

Detects CD34 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD34 antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD34 antibody. KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 monoclonal antibody (Catalog HB766107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD34 Recombinant Antibody (My10)