Anti-Human CD37 Recombinant Antibody (HH1) (HY564107)

Name: Anti-Human CD37 Recombinant Antibody (HH1)
Brand: abinScience
SKU: HY564107
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY564107
Reactivité des espèces	Human
Applications	ELISA, FCM, IF, WB
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	HH1
Applications testées	WB, FCM
Cible	CD37, Tspan-26, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P11049
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Leukocyte antigen CD37 (TSPAN26) is a ~31 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B. Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT. 1. Lapalombella, R. et al. (2012) Cancer cell 21, 694-708. PMID: 22624718 2. de Winde, CM. et al. (2016) The Journal of clinical investigation 126, 653-66. PMID: 26784544 3. Meyer-Wentrup, F. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 154-62. PMID: 17182550
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD37/TSPAN26 Antibody (HH1).
Flow Cytometry

Flow-cytometry using anti-human CD37 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD37 monoclonal antibody (Catalog HY564107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD37/TSPAN26 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 50ng
Lane 2: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 30ng
Lane 3: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 20ng