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| カタログ番号 | HY564107 |
|---|---|
| 種反応性 | Human |
| アプリケーション | ELISA, FCM, IF, WB |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | HH1 |
| テスト済みアプリケーション | WB, FCM |
| ターゲット | CD37, Tspan-26, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P11049 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | Leukocyte antigen CD37 (TSPAN26) is a ~31 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B. Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT. 1. Lapalombella, R. et al. (2012) Cancer cell 21, 694-708. PMID: 22624718 2. de Winde, CM. et al. (2016) The Journal of clinical investigation 126, 653-66. PMID: 26784544 3. Meyer-Wentrup, F. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 154-62. PMID: 17182550 |
| 注意事項 | For research use only. |
SEC-HPLC detection for Anti-Human CD37/TSPAN26 Antibody (HH1).
Flow-cytometry using anti-human CD37 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD37 monoclonal antibody (Catalog HY564107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-CD37/TSPAN26 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 50ng
Lane 2: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 30ng
Lane 3: recombinant human CD37/TSPAN26 (Catalog No: HY564011), 20ng
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