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Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549) (HV389023)

Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)
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概要
カタログ番号HV389023
説明
Anti-Human CD370/CLEC9A Nanobody (SAA2549) [SAA2549] (HV389023) is a alpaca monoclonal antibody detecting CLEC9A in ELISA, FCM. Suitable for Human.
Highlights
  • ●Flow Cytometry — Validated for flow cytometry applications.
種反応性Human
アプリケーションELISA, FCM
宿主種Alpaca
クローン性Monoclonal
アイソタイプVHH-8His-Cys-tag
クローンIDSAA2549
ターゲット C-type lectin domain family 9 member A, CD370, CLEC9A
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Purified by Nickel column.
アクセッション番号 Q6UXN8
形態 Liquid
保存バッファー 0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:2000-1:20000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

C-type lectin domain family 9 member A (CD370/CLEC9A) is a ~27 kDa protein. Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

注意事項 For research use only
イメージ
  • Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)

    SDS-PAGE

    SDS-PAGE for Anti-Human CD370/CLEC9A Nanobody (SAA2549)

  • Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)

    Bioactivity

    Detects recombinant CD370 (Catalog No: HV389011) in indirect ELISA.

  • Anti-Human CD370/CLEC9a Recombinant Nanobody (SAA2549)

    Immunofluorescence

    Immunofluorescence staining of MCF7 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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