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Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367) (HW564107)

Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)
Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)
Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)
Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)
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Aperçu
Numéro de catalogueHW564107
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneSRF367
Applications testées FCM
Cible Ecto-ATP diphosphohydrolase 1, NTPDase 1, ENTPD1, Ecto-ATPase 1, Ecto-apyrase, Ecto-ATPDase 1, Ectonucleoside triphosphate diphosphohydrolase 1, CD39, Lymphoid cell activation antigen
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P49961
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Ectonucleoside triphosphate diphosphohydrolase 1 (CD39/ENTPD1) is a ~57 kDa protein. Catalyzes the hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) (Probable). The enzyme sequentially removes phosphate groups in two successive steps, converting NTPs to nucleoside monophosphates (NMPs) via NDP intermediates (Probable). This activity contributes to the regulation of extracellular levels of nucleotides (Probable). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow.

1. Christoforidis, S. et al. (1995) European journal of biochemistry 234, 66-74. PMID: 8529670
2. Wang, TF. et al. (1996) The Journal of biological chemistry 271, 9898-901. PMID: 8626624
3. Kaczmarek, E. et al. (1996) The Journal of biological chemistry 271, 33116-22. PMID: 8955160
4. Robson, SC. et al. (1997) The Journal of experimental medicine 185, 153-63. PMID: 8996251
Note For research use only.
Images
  • Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD39/ENTPD1 Antibody (SRF367).

  • Anti-Human CD39/ENTPD1 Recombinant Antibody (SRF367)

    Flow Cytometry

    Flow-cytometry using anti-human CD39 antibody.CD39 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD39 antibody monoclonal antibody (Catalog # HW564107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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