Anti-Human CD3 epsilon Recombinant Antibody (SAA2371) (HY057053)

Name: Anti-Human CD3 epsilon Recombinant Antibody (SAA2371)
Brand: abinScience
SKU: HY057053
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY057053

Reactividad de especies

Human

Aplicaciones

ELISA, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG4, lambda

ID de clon

SAA2371

Aplicaciones probadas

Objetivo

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

Nivel de endotoxinas

< 10 EU/mg

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P07766

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

Nota

For research use only

Isotype Control

Human IgG4 (S228P), Lambda Recombinant Isotype Control Antibody (HyHEL-10) (HV799023)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human CD3E antibody (SAA2371)
Western blot

Western blot analysis was performed using anti-CD3E monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD3E (Catalog No: HY057021)
Lane 2: Negative control