Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057033)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Brand: abinScience
SKU: HY057033
Price: 370 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

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100ug
1mg

数量:

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY057033

説明

Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057033) is a human monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

種反応性

Human

アプリケーション

ELISA, FCM

宿主種

Human

クローン性

Monoclonal

アイソタイプ

VHH-hFc

クローンID

SAA2242

テスト済みアプリケーション

FCM

ターゲット

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

エンドトキシンレベル

< 10 EU/mg

純度

>95% as determined by SDS-PAGE.

精製

Purified by Nickel column.

アクセッション番号

P07766

形態

Liquid

保存バッファー

0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

注意事項

For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Flow cytometry

Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti- human CD3E monoclonal antibody (Catalog: HY057033,Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.