Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057043)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Brand: abinScience
SKU: HY057043
Price: 126 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY057043

説明

Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057043) is a alpaca monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

種反応性

Human

アプリケーション

ELISA, FCM

宿主種

Alpaca

クローン性

Monoclonal

アイソタイプ

VHH-His

クローンID

SAA2242

テスト済みアプリケーション

FCM

ターゲット

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

エンドトキシンレベル

< 10 EU/mg

純度

>95% as determined by SDS-PAGE.

精製

Purified by Nickel column.

アクセッション番号

P07766

形態

Liquid

保存バッファー

0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

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1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

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2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

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3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

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4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

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5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

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注意事項

For research use only.

イメージ

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.
Flow cytometry

Flow cytometry analysis was performed using anti-human CD3 monoclonal antibody. Jurkat cells were incubated with an irrelevant antibody (blue histogram) or FITC-conjugated anti-human CD3 monoclonal antibody (Catalog # HY057043, green histogram) at a concentration of 5 µg/mL for 30 min at room temperature. After washing, cells were directly analyzed on a NovoCyte Flow Cytometer.